anti phospho ask1 thr845  (Bioss)

Journal: Journal of Neuroinflammation

Article Title: Inhibition of apoptosis signal-regulating kinase by paeoniflorin attenuates neuroinflammation and ameliorates neuropathic pain

doi: 10.1186/s12974-019-1476-6

Figure Lengend Snippet: CCI induced neuropathic pain and increased the expression of p-ASK1 in the spinal cord of rats. a , b Mechanical allodynia and thermal hyperalgesia were significantly induced after CCI surgery ( n = 8). c The expression of p-ASK1 and ASK1 were significantly increased at day 14 after CCI surgery in the spinal cord of rats. The lumbar spinal cords (L1–L6) were collected and analyzed 14 days after the CCI operation ( n = 4). A significant difference was revealed following one-way ANOVA (* P < 0.05, ** P < 0.01, *** P < 0.001 vs. control; Bonferroni post hoc tests)

Article Snippet: Antibody for p-ASK1 (Thr845) (1:1000, bs-3031R) was purchased from Bioss (Woburn, MA).

Techniques: Expressing

Journal: Journal of Neuroinflammation

Article Title: Inhibition of apoptosis signal-regulating kinase by paeoniflorin attenuates neuroinflammation and ameliorates neuropathic pain

doi: 10.1186/s12974-019-1476-6

Figure Lengend Snippet: ASK1 inhibitor NQDI1 attenuated CCI-induced neuropathic pain and decreased the expression of p-p38 and p-JNK. a , b Single administration of ASK1 inhibitor NQDI1 (4 μg/20 μl, i.t.) at 14 days after CCI injury significantly attenuated CCI-induced mechanical allodynia and thermal hyperalgesia ( n = 8). c , d Consecutive administration of ASK1 inhibitor NQDI1 (4 μg/20 μl, i.t.) at days 14, 15, and 16 after CCI injury significantly attenuated CCI-induced mechanical allodynia and thermal hyperalgesia ( n = 8). e , f NQDI1 (4 μg/20 μl, i.t.) decreased the expression of p-JNK and p-p38 induced by CCI but did not affect p-ERK expression in the spinal cords of rats. The lumbar spines (L1–L6) were collected and analyzed 2 h after the drug administration ( n = 4). A significant difference was revealed following one-way ANOVA (* P < 0.05, ** P < 0.01, *** P < 0.001 vs. control; # P < 0.05, ## P < 0.01, ### P < 0.001 vs. CCI group; Bonferroni post hoc tests)

Article Snippet: Antibody for p-ASK1 (Thr845) (1:1000, bs-3031R) was purchased from Bioss (Woburn, MA).

Techniques: Expressing

Journal: Journal of Neuroinflammation

Article Title: Inhibition of apoptosis signal-regulating kinase by paeoniflorin attenuates neuroinflammation and ameliorates neuropathic pain

doi: 10.1186/s12974-019-1476-6

Figure Lengend Snippet: Paeoniflorin mimicked ASK1 inhibitor and decreased the phosphorylation of ASK1 in vivo. a AutoDock results show that paeoniflorin tightly bound to ASK1, which is equivalent to the ASK1 inhibitor NQDI1. b Paeoniflorin (20, 40, and 60 mg/kg, i.p.) significantly decreased the phosphorylation of ASK1 at 2 h after administration ( n = 4). The lumbar spines (L1–L6) were collected and analyzed 2 h after the drug administration. PF, paeoniflorin. Significant differences were revealed following one-way ANOVA (* P < 0.05, ** P < 0.01, *** P < 0.001 vs. control; # P < 0.05, ## P < 0.01, ### P < 0.001 vs. CCI group; Bonferroni post hoc tests)

Article Snippet: Antibody for p-ASK1 (Thr845) (1:1000, bs-3031R) was purchased from Bioss (Woburn, MA).

Techniques: In Vivo